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Plasmid Mapping Lab

Excel file to find the Best Fit line for Size Standard (right click to download the file)

Instructions on How to Write Discussion Section (right click to download the file)-

Peer Edit Sheet

Results:
-Paste in your gel photo (or the sample data) and label your lanes and the size standard lane.
-Include a title and a caption for it-- this is Figure 1. In the caption you should mention the run time (approximately 1 hour) and voltage for the gel (check your procedure) as well as the fact that it was a 0.8% agarose gel.

Conclusion: (Use the green packet to help you) GOES IN LAB NOTEBOOK
1) Match up the relevant lanes in the size standard (look for these 5: 6000, 5000, 4000, 3000, 2000). Click here for a sample.
2) Draw a zero line and determine the distance each size standard band travelled. Record this in a table (this should be Table 2-- don't forget the title and caption).
3) Put the numbers into the Excel program (see above for link) to get the equation that tells you the log(fragment size). The link to this program is above. Write the equation you get into the caption of Table 1.
4) Now measure the distance travelled by each of the fragments in the other lanes (your 4 samples) and record this in another table (Table 3). Use the yellow packet to help you set this table up).
5) Plug the distances into your equation to find out the sizes of all the fragments in step 4.
6) Use the information to draw plasmid maps of one plasmid. Use the fragments in the __0 samples to tell you what the uncut plasmid looks like-- you will ignore these fragments if you see them in the ___+ sample lane. This should be Figure 2. Again, don't forget the title and caption.

After you make your map, you will need to write the following to finish up your conclusion:
1) CER for map: Explain how you drew your map. Explain your thinking and logic.
2) Evaluation of evidence: why can you trust your data? Think about what you did and what evidence you have to show that the results you got can be trusted. Do not talk about execution errors here-- that is not the point of this section.
3) Next steps: What reasonable next steps could you take to build on the experiment that you did?

Video Demos:
Pipetting Small Volumes
Gel Loading

Lab Notebook Set-up:

1) Write a specific title (mention the 2 plasmids (B, pGLO or S)) as well as the restriction enzyme your group picks (HindIII or EcoRI).
2) Write an introduction.
3) Write who your lab partners are.
3) Paste in both pages of the procedure.